| 2026 | DigSeries | Elise | 55 |
| 2025 | SNL50: The Homecoming ConcertMovie | Self | 54 |
| 2025 | Good Hang with Amy PoehlerSeries | Self - Host | 54 |
| 2023 | Disney 100: Remember ThatMovie | Self | 52 |
| 2023 | First Time Female DirectorMovie | Meg | 52 |
| 2023 | Carol Burnett: 90 Years of Laughter + LoveMovie | Self - Guest | 52 |
| 2022 | Norman Lear: 100 Years of Music and LaughterMovie | Self | 51 |
| 2021 | MoxieMovie | Lisa Carter | 50 |
| 2020 | Stronger Together, Tous EnsembleMovie | Self | 49 |
| 2020 | DuncanvilleSeries | Duncan / Annie (voice) | 49 |
| 2020 | A Parks and Recreation SpecialMovie | Leslie Knope | 49 |
| 2020 | One World: Together at HomeMovie | Self | 49 |
| 2019 | The Kelly Clarkson ShowSeries | Self | 48 |
| 2019 | Desus & MeroSeries | Self - Illustrious Guest | 48 |
| 2019 | Sesame Street: 50 Years and CountingMovie | Self (archive footage) | 48 |
| 2018 | Love, GildaMovie | Self | 47 |
| 2017 | The HouseMovie | Kate Johansen | 46 |
| 2017 | Wet Hot American Summer: Ten Years LaterSeries | Susie | 46 |
| 2016 | WIRED's Autocomplete InterviewsSeries | Self - Guest | 45 |
| 2016 | Norman Lear: Just Another Version of YouMovie | Self | 45 |
| 2015 | Difficult PeopleSeries | Flute | 44 |
| 2015 | Wet Hot American Summer: First Day of CampSeries | Susie | 44 |
| 2015 | Inside OutMovie | Joy (voice) | 44 |
| 2015 | Riley's First Date?Movie | Joy (voice) | 44 |
| 2015 | Hurricane of Fun: The Making of Wet HotMovie | Self | 44 |
| 2015 | SistersMovie | Maura Ellis | 44 |
| 2015 | A Very Murray ChristmasMovie | Liz | 44 |
| 2015 | Thank You, Del: The Story of the Del Close MarathonMovie | Self | 44 |
| 2015 | Live from New York!Movie | Self | 44 |
| 2014 | The Tonight Show Starring Jimmy FallonSeries | Self | 43 |
| 2014 | Late Night with Seth MeyersSeries | Self - Guest | 43 |
| 2014 | They Came TogetherMovie | Molly | 43 |
| 2014 | Don Rickles: One Night OnlyMovie | Self | 43 |
| 2014 | Broad CitySeries | Cheryl | 43 |
| 2014 | Welcome to SwedenSeries | Amy Poehler | 43 |
| 2013 | Anchorman 2: The Legend ContinuesMovie | Wendy Van Peele (uncredited) | 42 |
| 2013 | Hollywood Game NightSeries | Self | 42 |
| 2013 | Free BirdsMovie | Jenny (voice) | 42 |
| 2013 | Are You HereMovie | Terri Baker | 42 |
| 2013 | A.C.O.D.Movie | Sondra | 42 |
| 2013 | The Last LegSeries | Self | 42 |
| 2012 | Comedy Bang! Bang!Series | Self | 41 |
| 2012 | The Greatest Event in Television HistorySeries | Jennifer Hart | 41 |
| 2011 | Fight for Your Right RevisitedMovie | Café Patron | 40 |
| 2011 | Alvin and the Chipmunks: ChipwreckedMovie | Eleanor (voice) | 40 |
| 2011 | Hoodwinked Too! Hood VS. EvilMovie | Gretel (voice) | 40 |
| 2010 | The Women of SNLMovie | Various (archive footage) | 39 |
| 2009 | Parks and RecreationSeries | Leslie Knope | 38 |
| 2009 | Monsters vs AliensMovie | Computer (voice) | 38 |
| 2009 | Late Night with Jimmy FallonSeries | Self | 38 |
| 2009 | The Jay Leno ShowSeries | Self | 38 |
| 2009 | The Tonight Show with Conan O'BrienSeries | Self - Guest | 38 |
| 2009 | Alvin and the Chipmunks: The SqueakquelMovie | Eleanor (voice) | 38 |
| 2009 | Saturday Night Live: The Best of Amy PoehlerMovie | Various (archive footage) | 38 |
| 2008 | Horton Hears a Who!Movie | Sally O'Malley (voice) | 37 |
| 2008 | Saturday Night Live Weekend Update ThursdaySeries | — | 37 |
| 2008 | Baby MamaMovie | Angie | 37 |
| 2007 | Blades of GloryMovie | Fairchild Van Waldenberg | 36 |
| 2007 | Shrek the ThirdMovie | Snow White (voice) | 36 |
| 2007 | The Graham Norton ShowSeries | Self | 36 |
| 2007 | Southland TalesMovie | Veronica Mung / Dream | 36 |
| 2007 | Mr. WoodcockMovie | Maggie | 36 |
| 2007 | Shortcut to HappinessMovie | Molly Gilchrest | 36 |
| 2007 | On BroadwayMovie | Farrah | 36 |
| 2007 | The Making of 'The Pick of Destiny'Movie | Self | 36 |
| 2007 | AsssscatMovie | — | 36 |
| 2006 | 30 RockSeries | Young Liz | 35 |
| 2006 | Tenacious D in The Pick of DestinyMovie | Truck Stop Waitress | 35 |
| 2006 | Man of the YearMovie | Amy Poehler | 35 |
| 2005 | Saving ManhattanMovie | Kristen | 34 |
| 2005 | Wonder ShowzenSeries | Miss Mary | 34 |
| 2004 | Mean GirlsMovie | Mrs. George | 33 |
| 2004 | EnvyMovie | Natalie Vanderpark | 33 |
| 2004 | O'GradySeries | — | 33 |
| 2003 | Arrested DevelopmentSeries | Gob's Wife | 32 |
| 2003 | Jimmy Kimmel Live!Series | Self | 32 |
| 2003 | The Ellen DeGeneres ShowSeries | Self | 32 |
| 2003 | Comedy Central RoastsSeries | Self | 32 |
| 2003 | Sick in the HeadMovie | — | 32 |
| 2001 | UndeclaredSeries | Hillary | 30 |
| 2001 | Wet Hot American SummerMovie | Susie | 30 |
| 1999 | SpongeBob SquarePantsSeries | Grandma (voice) | 28 |
| 1999 | Deuce Bigalow: Male GigoloMovie | Ruth | 28 |
| 1998 | Tomorrow NightMovie | Woman Getting Squirted | 27 |
| 1998 | Upright Citizens BrigadeSeries | Colby | 27 |
| 1997 | The ViewSeries | Self - Guest | 26 |
| 1996 | Spin CitySeries | Susan | 25 |
| 1992 | The Tonight Show with Jay LenoSeries | Self | 21 |
| 1989 | The SimpsonsSeries | Jenda (voice) | 18 |
| 1988 | LIVE with Kelly and MarkSeries | Self | 17 |
| 1983 | Friday Night VideosSeries | — | 12 |
| 1975 | Saturday Night LiveSeries | Self - Various Characters | 4 |
| 1975 | People's Choice AwardsSeries | Self - Presenter | 4 |
| 1953 | The OscarsSeries | Self - Presenter | — |
