| 2026 | For the Love of Star TrekMovie | Self | 94 |
| 2024 | Surge of Power: Where There's SmokeMovie | Omen | 92 |
| 2022 | Way of Tomorrow: The Evolution of Science Fiction MoviesMovie | Self - (archive footage) | 90 |
| 2021 | Star Trek: ProdigySeries | Uhura (voice) | 89 |
| 2020 | Challenger: The Final FlightSeries | Self (archive footage) | 88 |
| 2020 | Heaven's Gate: The Cult of CultsSeries | Self - Sister of Member (archive footage) | 88 |
| 2019 | Surge of DawnMovie | Omen | 87 |
| 2018 | United We FanMovie | Self | 86 |
| 2017 | Downward DogSeries | Deejay Devine | 85 |
| 2017 | Sharknado 5: Global SwarmingMovie | Secretary General of NATO | 85 |
| 2016 | 50 Years of Star TrekMovie | Self | 84 |
| 2016 | For the Love of SpockMovie | Self | 84 |
| 2015 | Roddenberry's TrekMovie | herself | 83 |
| 2014 | The Real History of Science FictionSeries | Self | 82 |
| 2014 | To Be TakeiMovie | Self | 82 |
| 2012 | Get a Life!Movie | Self | 80 |
| 2012 | Comic Book MenSeries | Self | 80 |
| 2010 | Scooby-Doo! Curse of the Lake MonsterMovie | Senator | 78 |
| 2009 | Bring Back... Star TrekMovie | Self | 77 |
| 2007 | Star Trek: Of Gods and MenMovie | Captain Nyota Uhura | 75 |
| 2007 | Star Trek: Beyond the Final FrontierMovie | Self | 75 |
| 2006 | HeroesSeries | Nana Dawson | 74 |
| 2006 | Comedy Central Roast of William ShatnerMovie | Self - Roaster | 74 |
| 2005 | Surge of Power: The Stuff of HeroesMovie | Oman | 73 |
| 2004 | Birth of a Timeless LegacyMovie | — | 72 |
| 2004 | Kiss 'N' Tell: Romance in the 23rd CenturyMovie | — | 72 |
| 2003 | Comedy Central RoastsSeries | Self | 71 |
| 2002 | Snow DogsMovie | Amelia | 70 |
| 2002 | After They Were FamousSeries | Self | 70 |
| 2000 | Buzz Lightyear of Star CommandSeries | Chief (voice) | 68 |
| 1999 | FuturamaSeries | Nichelle Nichols' Head (voice) | 67 |
| 1998 | Small Steps, Big Strides: The Black Experience in HollywoodMovie | Self | 66 |
| 1997 | TrekkiesMovie | Self | 65 |
| 1996 | Star Trek: 30 Years and BeyondMovie | Self | 64 |
| 1996 | Star Trek StoryMovie | Self | 64 |
| 1996 | William Shatner's Star Trek MemoriesMovie | Self | 64 |
| 1996 | The Last Angel of HistoryMovie | Self | 64 |
| 1995 | The Adventures of Captain Zoom in Outer SpaceMovie | Sagan | 63 |
| 1994 | Spider-ManSeries | Miriam (voice) | 62 |
| 1994 | GargoylesSeries | Diane Maza (voice) | 62 |
| 1993 | Star Trek: Deep Space NineSeries | Lt. Uhura | 61 |
| 1993 | Commander Toad in SpaceMovie | SS Stella (voice) | 61 |
| 1992 | Batman: The Animated SeriesSeries | Thoth Khepera (voice) | 60 |
| 1991 | Star Trek VI: The Undiscovered CountryMovie | Cmdr. Uhuru | 59 |
| 1989 | The SimpsonsSeries | Nichelle Nichols (voice) | 57 |
| 1989 | Star Trek V: The Final FrontierMovie | Cmdr. Uhura | 57 |
| 1986 | Star Trek IV: The Voyage HomeMovie | Cmdr. Uhura | 54 |
| 1984 | Star Trek III: The Search for SpockMovie | Cmdr. Uhura | 52 |
| 1984 | Goldene KameraSeries | Self | 52 |
| 1983 | Antony and CleopatraMovie | Charmain | 51 |
| 1982 | Star Trek II: The Wrath of KhanMovie | Cmdr. Uhura | 50 |
| 1979 | Star Trek: The Motion PictureMovie | Lt. Cmdr. Uhura | 47 |
| 1967 | Tarzan's Jungle RebellionMovie | Ruana | 35 |
| 1967 | Doctor, You've Got to Be Kidding!Movie | Jenny Ribbock | 35 |
| 1967 | Space SeedMovie | Uhura | 35 |
| 1966 | Star TrekSeries | Uhura | 34 |
| 1966 | Made in ParisMovie | Eleanor | 34 |
| 1966 | Mister BuddwingMovie | Dice Player | 34 |
| 1963 | The LieutenantSeries | Norma Bartlett | 31 |
| 1959 | Porgy and BessMovie | Dancer | 27 |